In this chapter, a detailed outline delineating the processing steps for microfabricating capillary electrophoresis (CE) with integrated electrochemical detection (ECD) platforms for performing analyte separation and detection is presented to enable
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Apr 25, 2016· The pelleted cells were resuspended with PBS buffer and disrupted by sonication on ice using ultrasonic cell crusher noise isolating chamber (XinChen biotechnology Co., LTD, China). The crude cell extract was separated by centrifugation. Afterward, the amount of GFP, as well as AP and NK, were determined by SDSPAGE according to standard ...
ultrasonic cell crusher noise isolating chamber (30 min). Then, ammonia solution ( mL, 25 wt%) was added as a catalyst, and the reacted mixture was vigorously stirred at room temperature. TEOS ( mL) was added 30 min later, and the reaction proceeded for 4 h. FITCAPTES ( mL) was then added into the mixture and stirred for another 1 h.